PUBLICATIONS

Books and Chapters
1. Spencer F T and Bruce I J (1989). Yeast Genetics: A Manual of Methods. Springer-Verlag, Berlin, Heidelberg and New York, ISBN3-540- 18805-3.
2. Bruce I J, Bruce A and Hewitt D (1998). Francesca's Story : Facilitating the earliest developments in 'Interaction in Action', 227-256, Hewitt D and Nind M. Eds. David Fulton Publishers Ltd, London. ISBN 1-85346-461-9.

Journals
3. Bruce I J and Wilkie D (1983). Selective mitochondrial activity distinguishes aspirin from salicylate and benzoate in yeast cells. Biochem. Pharmacol., 32, 2113-2116.
4. Bruce I J and Wilkie D (1984). Effect of aspirin on mitochondrial mutagens and oligomycin in Saccharomyces cerevisiae. Heredity, 52, 454-455.
5. Bruce I J and Wilkie D (1984). Effect of aspirin on mitochondrial mutagens in Saccharomyces cerevisiae. Mol. Gen. Genet., 194, 299-302.
6. Bruce I J, Hardy J and Stacey K A (1984). Potentiation by purines of the growth inhibitory effects of sulphonamides on E. coli K12 and the location of the gene which mediates this effect. J. Gen. Microbiol., 130, 2489-2495.
7. Bruce I J, Evans I H and Wilkie D (1987). The yeast mitochondrial system as a basis for carcinogen testing. J. Appl. Bacteriol. Technical Handbook series (23), 109-135.
8. Bruce I J and Kerry R (1987). The effect of chloramphenicol on platelet protein synthesis and aggregation. Biochem. Pharmacol., 36, 1769-1775.
9. Henderson G E, Evans I H and Bruce I J (1988). Vanadate inhibition of mitochondrial respiration and H+ATPase activity in Saccharomyces cerevisiae. Yeast, 5, 73-77.
10. Henderson G E, Evans I H and Bruce I J (1989). The effects of vanadate on yeast, Saccharomyces cerevisiae. Antonie van Leeuwenhoek, 55, 99-107.
11. Spencer J F, Spencer D M, Reynolds N C, Cromie R L and Bruce I J (1989). Complementation of auxotrophic requirements in a diploid strain of Saccharomyces cerevisiae by protoplast fusion with protoplasts of Hansenula wingei. Yeast, 5 Spec.No: S293-6.
12. Bruce I J and Metherell L. (1994). Experiments to determine the importance and desirability of a 4°C hold temperature at the end of thermal cycling. Biomedical Products, 19, 60.
13. Bartlett T J, Aibangbee A, Bruce I J, Donovan P J and Yon R (1994). Endogenous polypeptide-chain length and partial sequence of aspartate transcarbamoylase from wheat, characterised by immunochemical and cDNA methods. Biochim. Biophys. Acta., 1207 187-193.
14. Bruce I J (1994). Nucleic acid amplification mediated microbial identification. Science Progress, 77 (3/4), 183-206.
15. Smethurst D, Davies M and Bruce I J (1994). Magnetic solid phase supports for affinity purification of nucleic acids. in Separation for Biotechnology Ed D.L. Pyle pp152-158. Royal Society of Chemistry, London. UK.
16. Ray R, Smith M, Simm R, Bruce I and Wakefield A (1995) In situ hybridisation detection of short viral amplicon sequences within cultured cells and body fluids after the in situ polymerase chain reaction. J. Virol. Meths., 52, 247-263.
17. Metherell L, and Bruce I J (1995) Single and multiplex bacterial identification using restriction endonuclease target sequences. Int. Biotechnol. Lab., 13 (5), 20-21.
18. Bruce I J, Davies M J, Howard K, Smethurst D E and Todd M (1996) Magnetisable solid phase supports for purification of nucleic acids. J. Pharm. Pharmacol., 48, 147-149.
19. Xia X, Oswiecimska K, Aathithan A, Smith A R W and Bruce I J (1996) Characterisation and sequencing of a plasmid encoding 2,4-D dissimilation. BiotechLab International, 2, 18-19.
20. Bignell G R, Bruce I J and Evans I H (1996) Electrophoretic karyotype of the amylolytic yeast Lipomyces starkeyi and cloning, sequencing and chromosomal localisation of its TRP 1 gene. Curr. Genet., 30, 83-88.
21. Xia X, Aathithan S, Smith A R W and Bruce I J (1996). Identification and sequencing of a novel insertion sequence, IS1471, in Burkholderia cepacia strain 2a. FEMS Microbiol. Letts., 144, 203-206.
22. Smethurst D, Davies M, Howard K, Todd M and Bruce I J (1997). Improved Manufacture and Application of an Agarose Magnetisable Solid-Phase Support. Appl. Biochem. Biotechnol., 68, 95-112.
23. Metherell L, Hurst C, and Bruce I J (1997). Rapid, sensitive, microbial detection by gene amplification using restriction endonuclease sequences. Mol. Cell. Probes., 11, 297-308.
24. Chadwick N, Bruce I J, Davies M, Gemen B, Schukkink R, Khan K, Pounder R, Wakefield A. (1998). A sensitive and robust method of measles RNA detection. J. Virol. Meths., 70, 59-70.
25. Xia X, Oswiecimska K, Aathithan, A, Smith, A R W and Bruce, I J (1998). A novel plasmid pIJB1 possessing a putative 2,4-dichlorophenoxyacetate degradative transposon Tn5530 in Burkholderia cepacia strain 2a. Plasmid, 39, 154-159.
26. Chadwick N, Bruce I J, Schepelmann S, Pounder R E and Wakefield A J (1998). Measles virus RNA is not detected in Inflammatory Bowel Disease using hybrid capture and reverse transcription followed by the polymerase chain reaction. J. Med. Virol., 55, 305-311.
27. Chadwick N, Wakefield A J, Pounder R E, Bruce I J, (1998). A comparison of the relative fidelities of three RNA amplification methods as a source of DNA for sequencing. Biotechniques, 25, 818-822.
28. Levison P R, Badger S E, Dennis J, Hathi P, Davies M J, Bruce I J and Schimkat D (1998). Recent developments of magnetic beads for use in nucleic acid purification. J. Chromatogr. A, 816, 107-111.
29. Davies M J, Taylor J I, Sachsinger N and Bruce I J (1998). Isolation of plasmid DNA using magnetite as a solid phase adsorbent. Anal. Biochem., 262, 92 - 94.
30. Levison P R, Badger S E, Hathi P, Davies M J, Bruce I J and Grimm V (1998). New approaches to the isolation of DNA by ion exchange chromatography. J. Chromatogr. A, 827, 337-344.
31. Hurst C D, Knight A and Bruce I J (1999). PCR detection of genetically modified soya and maize in foodstuffs. Mol. Breed. 5, 578-586.
32. Hurst C D, Bartlett S E, Davidson W S and Bruce I J. (1999). The complete mitochondrial DNA sequence of the Atlantic salmon, Salmo salar. Gene, 239, 237-242.
33. Davies M J, Shah A and Bruce I J (2000). Synthesis and application of fluorescently labelled oligonucleotides and nucleic acids. Chem. Soc. Review., 29, 97-107.
34. Taylor J I, Hurst C D, Davies M J, Sachsinger and Bruce I J. (2000) Application of magnetite and silica-magnetite composites to the isolation of genomic DNA. J. Chromatogr. A, 890, 159-166
35. Bignell G R, Bruce I J and Evans I H (2000) Amylolytic enzymes of the yeast Lypomyces starkeyii: purification and size determination. Biotechnol. Letts., 22, 1713-1718
36. Poh R, Xia X, Bruce I J and Smith A R W (2001) 2,4-Dichlorophenoxyacetate/a-ketoglutarate dioxygenases from Burkholderia cepacia 2a and Ralstonia eutropha JMP134. Microbios, 105, 43-63
37. Poh R, Smith A R W and Bruce I J (2002) Complete characterisation of Tn5530 from pIJB1 in Burkholderia cepacia strain 2a. Plasmid, 48, 1-12.
38. Yiu H P and Bruce I J (2003) Biological applications of organically functionalised mesoporous molecular sieves and related materials. Studies in surface science and catalysis, 146, 57-60.
39. Yiu H P, Bruce I J, McGuiness F and Wright, P A (2003) A novel preparation route for palladium-carbon composite materials-pore filling of SBA-15 mesoporous molecular sieve. Studies in surface science and catalysis, 146, 581-584.
40. Sanden M, Bruce IJ, Rahman MA and Herme G-I (2003) The fate of transgene sequences present in genetically modified (GM) plant products in fish feed: Investigating the survival of GM Soybean DNA fragments during feeding trials in Atlantic salmon, Salmo salar. Mol. Breed. (in press)
41. Amagliani G, Brandi G, Omiccioli E, Casiere A, Bruce IJ , Magnani M (2003) Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and PCR. (in press)
42. Bertozzini E, Penna A, Pierboni E, Bruce IJ and Magnani M (2003) Development of new procedures for the isolation of phytoplankton DNA from fixed samples. Marine Biotechnology (in press)
43. Lellouche J-P, Perlman N, joseph A, Govindaraji S, Buzhansky L, Yakir A and Bruce I (2004) New magnetically responsive polycarbazole-magnetite nanoparticles. Chem. Commun, 560-561
44. Carrington B, Bruce I J, Smith A R W S (2003) Affinity purification and biochemical characterisation of chlorophenol hydroxylases from Burkholderia cepacia strain 2a, Alcaligenes eutrophus JMP134 and Pseudomonas NCIMB 9340 Microbiology (in preparation).
45. King K, Hurst C, Rahman A, Bruce I J (2003) Study of differential gene expression in bacteria using aligned cell cultures and RAP-PCR. J. Micro. Meths. (submitted)
46. Bruce IJ, Taylor JI, Todd M, Davies MJ and Sen T (2003) The synthesis, characterisation and application of silica-magnetite nanocomposites. (submitted)

Refereed Abstracts and Published Presentations
47. Henderson G, Bruce I J, Evans I H, Patel B and Haswell S (1986). Some preliminary studies of the effect of vanadate on yeast (S. cerevisiae) cell function. Society for General Microbiology 107th Meeting, Swansea, Abstracts p. 9.
48. Aathithan S, Smith A R W and Bruce I J (1986). Physical characterisation of a plasmid involved in the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) XIV International Conference for Microbiology, Manchester University. Acinetobacter satellite group meeting, Abstracts p. 8.
49. Spencer J F T, Spencer D M, Reynolds N, Cromie R and Bruce I J (1988). Complementation of auxotrophic requirements in a diploid strain of Saccharomyces cerevisiae, by fusion with protoplasts of Hansenula wingei and Zygosaccharomyces rouxii. 7th International Symposium on Yeasts, Perugia,Italy, Abstracts p. 97.
50. Henderson G E, Evans I H and Bruce I J (1989). Inhibition of mitochondrial H+ATPase activity in yeast, Saccharomyces cerevisiae by vanadate. 14th Int. Conf. on Yeast Genetics and Molecular Biology, Helsinki, Finland, Yeast, 4, Abstract S224.
51. Carrington B, Bruce I J, Smith A R W and Yon R J. (1990) Affinity purification and biochemical characterisation of a 2,4-dichlorophenol hydroxylase from Pseudomanas cepacia 2a. 5th European Congress for Biotechnology, Copenhagen, Denmark, July 1990. Abstract p.218
52. Carrington B, Bruce I J, Smith A R W and Hartwell R. (1990) Amino acid analysis and sequencing of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia 2a. 5th European Congress for Biotechnology, Copenhagen, Denmark, July 1990. Abstract p. 217.
53. Aathithan S, Bruce I J, Maclean K and Yon R J. (1990) Physical characterisation of an 88 kilobase degradative plasmid possessed by Pseudomonas cepacia 2a. 5th European Congress for Biotechnology Copenhagen, Denmark, July 1990. Abstract p. 215.
54. Maclean K, Bruce I J and Aathithan S. (1990) Identification, cloning, and expression of p 2,4-D genes involved in 2,4-dichlorophenoxyacetate (2,4-D) dissimilation. 5th European Congress for Biotechnology, Copenhagen, Denmark, July 1990. Abstract p. 219.
55. Bruce I J, Aathithan S, Carrington B and Maclean K (1990). Pseudomonas cepacia and 2,4 dichlorophenoxyacetate dissimilation. 5th European Congress for Biochemistry, Copenhagen, Denmark, July 1990. Abstracts.
56. Carrington B and Bruce I J (1990). Properties of chloroaromatic hydroxylases from P. cepacia 2a, Alacaligens eutraphus and Pseudomonas NCIMB 9340. International Conference of Flavins and Flavoproteins, Como, Italy, August 1990. Abstract p. 57.
57. Bartlett T, Bruce I J and Yon R (1991). A partial sequence for aspartate transcarbamoylase from wheat (Triticum aestivum). ATCase Workshop, Penn. State University, Penn. State, USA, May 1991
58. Maclean K, McClure N and Bruce I J (1991). Sequencing and expression of dissimilatory genes possessed by p 2,4D in P. cepacia strain 2a. Symposium of biotechnology in the environment. Sustainable development (UNESCO and MIRCEN) Montreal, Canada, September 1991
59. Bartlett T J, Bruce I J and Yon R J (1991). Partial Primary structure of aspartate transcarbamoylase from wheat. Biochemical Society, (December), London.
60. Bartlett T J, Bruce I J and Yon R J (1992). A partial sequence for aspartate transcarbamoylase from wheat. Sixth Harden Satellite Meeting, The Genes and Enzymes of pyrimidine bisynthesis II, Glasgow, Scotland, Sept. 1992.
61. Hayman E, Reynolds K M and Bruce I J (1992). Novel purification scheme for Taq Polymerase and some biochemical and biophysical enzyme studies. Thermophiles: Science and Technology, Reykjavik, Iceland, August 1992. Abstract p. 121.
62. Maclean K, Barnard R, Bustin S, McClure N and Bruce I J (1993). Physical and genetic characterisation of a plasmid possessed by Pseudomonas cepacia strain 2a involved in 2,4-dichlorophenoxyacetate dissimilation. Sixth European Congress on Biotechnology, Florence, Italy, June 1993. Abstract TH120.
63. Xia X, Bruce I J, Smith A R W and Maclean K (1993). Studies on the ether cleavage enzyme and its gene involved in 2,4-dichlorophenoxyacetate dissimilation in Pseudomonas cepacia strain 2a. Sixth European Congress on Biotechnology, Florence, Italy, June 1993. Abstract TH153.
64. Smethurst D E, Howard K and Bruce I J (1993). Nucleic acid separation and purification using a magnetisable solid phase support (MSPS). Sixth European Congress on Biotechnology, Florence, Italy. June 1993. Abstract TU413
65. Metherell L A and Bruce I J (1993). Rapid Microbial Identification. Sixth European Congress on Biotechnology, Florence, Italy. June 1993. Abstract WE322.
66. Davies M J and Bruce I J (1993). Magnetic solid phase supports for affinity purification of nucleic acids. Sixth European Congress on Biotechnology, Florence, Italy. June 1993. Abstract M0098 and M0099.
67. Hayman E V, Howard K M and Bruce I J (1993). Purification scheme for a DNA polymerase from Thermus aquaticus YT1. Sixth European Congress on Biotechnology Florence, Italy. June 1993. Abstract M0130.
68. Fedorec A M, Rennolls K and Bruce I J (1994). Model based control and decision management in the PCR laboratory. 7th International Congress of Bacteriology and Applied Microbiology, Prague, Czech Republic. July 1994 Abstract p316.
69. Metherell L and Bruce I J (1994). Rapid, sensitive microbial identification by gene amplification using a novel class of target sequence. 7th International Congress of Bacteriology and Applied Microbiology, Prague, Czech Republic. July 1994 Abstract p316.
70. Bruce I J (1994). PCR product detection and characterisation in diagnostics. Optical Sensors in Medicine, Manchester, England June 1994 Abstract p.8.
71. Bruce I J, Davies M J, Howard K M, Smethurst D E and Todd M (1994) Separation and purification of nucleic acids using a magnetisable solid phase support. 2nd International Symposium on Medicinal Chemistry, Bath, UK. September 1994. Abstract No.6.
72. Metherell L and Bruce I J. (1995) Rapid, sensitive, microbial detection using restriction endonuclease target sequences. New Applications of Emerging Molecular Diagnostics for Infectious Diseases. London UK, July 1995 Abstract, p26.
73. Davies M J, Bruce I J and Higgins L M (1995) Application of magnetisable solid phase supports to the generation and screening of combinatorial chemical libraries. Combinatorial Synthesis Symposium, Exeter, UK. July 1995 Abstract p2.
74. Bruce I J, (1996) Nucleic acid amplification : the past, present and future. Society for Industrial Microbiology. Annual Meeting, Research Triangle Park, North Carolina, USA. August 1996.
75. Chadwick N, Bruce I J, Davies M, Pounder R and Wakefield A (1997) Hybrid capture followed by RT-PCR does not detect measles RNA in intestinal tissue. Gut, 40 (S1), TH111.
76. Chadwick N, Bruce I J, Davies M, Pounder R and Wakefield A (1997) Hybrid capture followed by RT-PCR does not detect measles RNA in intestinal tissue. Gastroenterology 112, No 4 SS, p.A946.
77. Hathi P, Davies M J, Bruce I J, Muller H N, Badger S E, Levison P R and Dennis J (1997). Recent developments in the isolation of plasmid DNA - a comparative study. 17th International Symposium on the separation of proteins peptides and polynucleotides. Rochville, Mayford, USA. Abstract L306, p51.
78. Levison P R, Dennis J, Badger S E, Hathi P, Davies M J, Bruce I J and Schmikat D (1998). New approaches to the isolation of plasmid DNA by ion-exchange chromatography. PREP '98, Washington, USA, June 1998. Abstract P130, p50.
79. Bruce I J (1998) 'Teaching Company Scheme Generates Magnetic Attraction' in Innovation in BioSciences, BBSRC Annual Conference, QE2 Centre, London, October 1998. pp10
80. Shah A, Davies M J and Bruce I J (1999). The Synthesis of Multiply, Fluorescently Labelled Oligonucleotides. The Fourth International Symposium on Functional Dyes. Osaka, Japan June1999 Abstract PA008, p34.
81. Taylor J I, Hurst C D, Davies M J, Bruce I J and Sachsinger N. (1999). Application of magnetite and silica magnetite composites to DNA isolation. 19th International Symposium the purification of proteins, peptides and polynucleotides. Florida, USA. October 1999. Abstract 105, p37
82. Shah A, Davies M J and Bruce I J (2000). The synthesis of multiply, fluorescently - labelled oligonucleotides. 11th Royal Australian Chemical Institute Convention (RACIC): Division of Organic Chemistry Programme. Australia National University Canberra, Australia. February 2000. Abstract P99, p157
83. Davies M J, Bruce I J and Anson D K A (2000) Molecular imprint polymers of biological macromolecules in an aqueous environment. 1st International Workshop on Molecular Imprinting, Cardiff, UK. July 2000. Abstract p50.
84. Poh R, Smith A R W and Bruce I J (2000) Characterisation of the putative transposon Tn5530 in Burkholderia cepacia strain 2a. Fourteenth forum for Applied Biotechnology, University of Gent, Belgium, September 2000. Abstract p115 - 118
85. Codd J D, Hurst, C D, Bruce I J and Salisbury J R (2001) An Immuno-cytochemical study of potential oncogenes in transformation of follicular lymphoma. Joint meeting of the Pathological Society of Great Britain and Ireland with the Dutch Pathological Society, University of Maastricht, Netherlands. January 2001, Abstract 72
86. Fedorec A, Blackshields D, Reynolds K and Bruce I J (2001) Intelligent instrumentation and control for optimising DNA amplification. Istitut Teknolog Bandng, Indonesia. July 2001. Proceedings p 89-96
87. Yiu H P and Bruce I J (2002) Biological applications of organically functionalised mesoporous moleculr sieves and related materials. 3rd International Mesostructured Materials Symposium, Jeju, Korea. July 2002, Abstract OP-16, p28
88. Yiu H P, Bruce I J, McGuiness F and Wright P (2002) A novel preparation route for palladium-carbon composite materials-pore filling of SBA15 mesoporous molecular sieve. 3rd International Mesostructured Materials Symposium, Jeju, Korea. July 2002, Abstract PA-8, p58
89. Bruce, IJ (2002) Innovative DNA chips, applications for food safety, civil security etc. European Research 2002 Conference. Brussels, Belgium, November 2002 (Plenary speaker)
90. Bruce, IJ (2002) Paramagnetic silica, agarose and mesoporous molecular sieves: new materials and applications in biomolecular separations. National Science Foundation/European Commission Workshop: From Nanomaterials to Nanotechnology. Boston, USA, December 2002 (EU invited speaker)
91. Bruce, IJ (2002) Paramagnetic silica, agarose and mesoporous molecular sieves: new materials and applications in biomolecular separations. EU-China forum on nanosized technology. Beijing, China, December 2002 (EU invited speaker)
92. Bruce, IJ (2003) Paramagnetic silica, agarose and mesoporous molecular sieves: new materials together with novel surface activation and DNA modification chemistries for applications in biomolecular separations. VI Congresso Nazionale di Chimica Supramoleculare, Urbino, Italy. July 2003, Abstract L5, p12
93. Bruce, IJ (2003) Synthesis and characterisation of silica-magnetite hybrid composites. EU-China Workshop on Multifunctional Materials by Design, Bologna, Italy, October 2003, Bologna, Italy, (EU invited speaker), Abstract 18.
94. Bruce, IJ (2004) Key for tomorrow:nanotechnology in food ananlysis. Rapid Methods Europe 2004, Amsterdam, Holland. March 2004. Abstract p.

Patents and applications
95. Magnetic solid phase supports: Magnetic separation. 6th November 1992 with Hybaid Ltd. pat. appl. no. 92/23334.5.
96. Microbiological Detection. 25th June 1993 with Techne (Cambridge) Ltd. pat. appl. no. 93/13150.6.
97. Capture of single stranded nucleic acids. 5th July 1996 with Molecular Sciences Ltd. pat. appl. no. 08/866,446
98. Capture of single stranded nucleic acids. 20th March 2001 with Molecular Sciences Ltd. US pat. no 6,203,978
99. Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes.19th October 2001 with Proligo GmbH. pat. appl. no. 60/336,432

Nucleotide SequencesGenBank
Accession number Z68292 1996 - L.Starkeyi trp1 geneEMBL
Accession number U67938 1997 - B.cepacia insertion sequence IS1471 putative transposase (tnp1471) gene, complete sequenceEMBL
Accession number U87394 1997 - B.cepacia plasmid pIJB1, 2,4-D dioxygenase (ijbA) mRNA, complete sequenceGenBank
Accession number AF029344 1999 - B.cepacia plasmid pIJB1, 2,4 - dichlorophenol hydroxylase (ijbB) gene, partial cdsGenBank
Accession number AF029344 1999 - B.cepacia plasmid pIJB1, 2,4 - dichlorophenol hydroxylase (ijbB) gene, partial cdsGenBank
Accession number AF029344 2001 - Entire Tn5530 sequence indicating coding regions and operon structures GenBank
Accession number U12143 1999 - Salmo salar mitochondrion, complete genome