APPLIED CYTOMETRY
CITOMETRIA APPLICATA
Applied Cytometry
Citometria applicata / Applied Cytometry
| A.Y. | Credits |
|---|---|
| 2015/2016 | 6 |
| Lecturer | Office hours for students | |
|---|---|---|
| Loris Zamai |
Assigned to the Degree Course
| Date | Time | Classroom / Location |
|---|
Learning Objectives
The course aims to give students knowledge of the main flow cytometric techniques applied to several aspects of cell biology. In particular, the methodologies to study the cell survival and death (apoptosis and necrosis), the cell cycle, the immunophenotyping, the cell proliferation and differentiation and some cell functions. After the acquisition of the basic information regarding data analysis, the flow cytometric approach will be compared with the standard methodologies of microscopy and biochemistry.
Program
Analyses of cell survival and death. Evaluation of cell apoptosis and necrosis. Apoptosis/necrosis progression. Analysis of DNA content. Analysis of cell cycle with mono-parametric (propidium iodide, PI) and bi-parametric (PI/BrdU) approaches. Evaluation of the frequency of cell mutants by flow cytometry. Analysis of phenotype and function of the immune system. Analysis of intracytoplasmic proteins (cytokines and onco-proteins) and of cytotoxic activity of some cells of peripheral blood samples. Absolute count of haematopoietic progenitors. Hematopoietic differentiation in vitro. Use of monoclonal antibodies against surface antigens in the describtion of haematopoietic cell differentiation. Subsets of peripheral blood, phenotype and function.
Learning Achievements (Dublin Descriptors)
IN AGREEMENT WITH THE DUBLIN'S DESCRIPTORS, AT THE END OF THE COURSE, THE STUDENT WILL HAVE TO PRESENT THE TOPICS IN A CRITICAL MANNER AND WITH AN APPOPRIATE TERMINOLOGY
Teaching Material
The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it
Supporting Activities
Data analysis with cytofluorimetric softwares.
Teaching, Attendance, Course Books and Assessment
- Teaching
The course will consists in lecturers and lab experiences.
- Course books
Dispensa di citometria applicata. L.Zamai et al. presso Fabbri Luca Copisteria, Centro Copie, Via Saffi 50, Urbino e materiale didattico on-line.
Citometria Applicata. L.Zamai et al. Fabbri Luca Copisteria, Centro Copie, Via Saffi 50, Urbino and slides of the lessons in pdf format (WEB page)
- Assessment
Oral examination (can be either in English or Italian)
- Disabilità e DSA
Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.
A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.
Additional Information for Non-Attending Students
- Teaching
Part-time
WEB page with lessons in pdf format
- Course books
Part-time
Citometria applicata. L.Zamai et al. Fabbri Luca Copisteria, Centro Copie, Via Saffi 50, Urbino and slides of the lessons in pdf format (WEB page)
- Assessment
Oral examination (can be either in English or Italian)
- Disabilità e DSA
Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.
A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.
Notes
The student can request to sit the final exam in English with an alternative bibliography.
| « back | Last update: 10/06/2016 |
