Università degli Studi di Urbino Carlo Bo / Portale Web di Ateneo


ADVANCED BIOLOGICAL TECHNOLOGIES
TECNOLOGIE BIOLOGICHE AVANZATE

A.Y. Credits
2016/2017 6
Lecturer Email Office hours for students
Luca Galluzzi
Teaching in foreign languages
Course with optional materials in a foreign language English
This course is entirely taught in Italian. Study materials can be provided in the foreign language and the final exam can be taken in the foreign language.

Assigned to the Degree Course

Sanitary Nutritional and Environmental Biology (LM-6)
Curriculum: DIAGNOSTICA MOLECOLARE
Giorno Orario Aula

Learning Objectives

The aim of the course is to provide notions of advanced biological technologies such as quantitative PCR, DNA microarrays, protein microarrays, miRNA microarrays, comparative genomic hybridization (CGH), Chromatin immunoprecipitation (ChIP)-on-Chip, Next-generation sequencing and genome editing. Notions of bioinformatics for the genome analysis and the search of biological databases will also be acquired.

Program

  • Main tools for DNA/protein sequence analysis and for searching biological information in public databases (e.g. BLAST, PubMed, etc.)
  • Techniques for nucleic acid amplification: PCR-based techniques; Non PCR-based techniques; Whole Genome Amplification; Whole Transcriptome Amplification. 
  • Real-time PCR: principles, methods, applications and data analysis.
  • High Resolution Melt (HRM) analysis: principles and examples of applications.
  • DNA Microarrays: manufacturing principles; planar microarrays; bead microarrays; applications in transcriptome analysis and in molecular diagnostics (SNP arrays, CGH arrays, arrays for the detection and characterization of microorganisms.
  • Protein arrays: function arrays; detection arrays; reverse phase arrays.
  • RNA interference: techniques to study microRNAs; miRNA-mediated gene silencing
  • Chip-on-chip: methods and applications to study transcription factors and chromatin organization.
  • Next-generation sequencing technologies based on pyrosequencing, reversible terminators, oligonucleotide ligation detection (SOLiD), hydrogen ion detection (ion torrent).
  • Metagenomics: applications for the analysis of microbial communities.
  • Genome engineering through CRISPR/Cas-based methods.
     
  • Learning Achievements (Dublin Descriptors)

    D1 – Knowledge and ability of comprehension. The student must demonstrate to possess solid knowledge of basic molecular biology and good knowledge of molecular technologies teached during the course. 

    D2 – Ability to apply knowledge and comprehension. The student must be able to implement/use these knowledges for the application in the research and/or diagnostics fields. 

    D3 – Autonomy of judgement. The student will be able to critically evaluate the methods/strategies used for diangostics or research applications. 

    D4 – Communication skills. The student must acquire adequate scientific language skills, and he must have a participatory and critical attitude to the topics of the lectures.

    D5 – Learning skill. The student will be able to build his own learning path, using learning materials furnished by the teacher or autonomously acquired.

    Teaching Material

    The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

    Supporting Activities

    Search for biological information in public databases.


    Didactics, Attendance, Course Books and Assessment

    Didactics

    Lectures

    Attendance

    None

    Course books
  • Molecular Diagnostics, 2nd Edition 2009 Patrinos, Ansorge. Elsevier academic press. 
  • ppt files and scientific reviews published on international journals will be made available online (moodel platform)
  • Assessment

    Oral examination. During the examination the student must demonstrate: that it has acquired full mastery of the concepts and technologies covered during the course; to be able to make logical connections between the techniques learned and their applications; to be able to illustrate the key concepts in a limited time and using appropriate scientific language.

    Additional Information for Non-Attending Students

    Course books
  • Molecular Diagnostics, 2nd Edition 2009 Patrinos, Ansorge. Elsevier academic press. 
  • ppt files and scientific reviews published on international journals will be made available online (moodel platform)
  • Assessment

    Oral examination. During the examination the student must demonstrate: that it has acquired full mastery of the concepts and technologies covered during the course; to be able to make logical connections between the techniques learned and their applications; to be able to illustrate the key concepts in a limited time and using appropriate scientific language.

    « back Last update: 27/04/17

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