Università degli Studi di Urbino Carlo Bo / Portale Web di Ateneo


BIOCHEMICAL METHODOLOGY
METODOLOGIE BIOCHIMICHE

A.Y. Credits
2017/2018 8
Lecturer Email Office hours for students
Ferdinando Mannello Do not hesitate to contact the Teacher by Lab phone (0722304621) or mobile (329-3610287) from Monday to Friday
Teaching in foreign languages
Course with optional materials in a foreign language English
This course is entirely taught in Italian. Study materials can be provided in the foreign language and the final exam can be taken in the foreign language.

Assigned to the Degree Course

Biological Sciences (L-13)
Curriculum: SANITARIO MOLECOLARE
Date Time Classroom / Location
Date Time Classroom / Location

Learning Objectives

The aims of the course is to provide students with the tools  to understand the methods for the study of proteins (enzymes) and metabolites. With the lab experiences, the students will be able to assay the activity of an enzyme, to purify a protein and measure the concentration of a metabolite as well as to perform an electrophoresis.

Program

1) Principles of biochemical methods

2) Main tools/instruments of biochemical laboratories

3) Solutions and buffers

4) Prein purification

5) Chromatography

6) Electrophoresis

7)Quantitative assays

8) Immunchemical assays

9) Enzymatic kinetic 

(see italian program for more details)

Bridging Courses

It is  suggested to have previously performed and passed the exam of Biochemistry (second year) to improve the basis knowledge

Learning Achievements (Dublin Descriptors)

The Dublin Descriptors will be the teaching goals 

Teaching Material

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Supporting Activities

Students will perform the following lab experiments with the collaboration of Dr. Ligi and  Dr. Croce : protein electrophoresis, zymography, mini-spin chromatography, immunomagnetic assay. The students will receive the protocols of the lab experiments.

 


Didactics, Attendance, Course Books and Assessment

Didactics

Lessons and laboratory seminar activities

Attendance

Attendance of lectures is not mandatory, but students are warmly requested to participate in the lab experiences

Course books

Maria Carmela Bonaccorsi di Patti, Roberto Contestabile, Martino Luigi Di Salvo: Metodologie biochimiche. Casa Editrice Ambrosiana.

Carlo De Marco, Chiara Cini: Principi di metodologia biochimica. Casa Editrice Piccin.

Assessment

Oral exam

Additional Information for Non-Attending Students

Didactics

The same as for attending students

Attendance

The same as for attending students

Course books

The same as for attending students

Assessment

The same as for attending students

Notes

For further informations, please contact the Teacher via mail or by phone at +39-0722-304621

« back Last update: 27/07/2017

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