Università degli Studi di Urbino Carlo Bo / Portale Web di Ateneo


A.Y. Credits
2019/2020 4
Lecturer Email Office hours for students
Claudio Ortolani Students will be received on tuesady after the end of the last lesson. The scheduled time is half an hour.
Teaching in foreign languages
Course with optional materials in a foreign language English
This course is entirely taught in Italian. Study materials can be provided in the foreign language and the final exam can be taken in the foreign language.

Assigned to the Degree Course

Sanitary Nutritional and Environmental Biology (LM-6)
Date Time Classroom / Location
Date Time Classroom / Location

Learning Objectives

To transmit information useful to independently deal with the cytometric approach to the diagnosis of the most frequent haematological and non haematological diseases that can be routinely encountered in the Clinical Pathology laboratory


Development of the following topicts through direct computer emulation

Overview of cytometric data analysis

The "Lymphocyte typing" exam; execution,appropriateness, and meaning

Reactive lymphocytosis and neoplastic lymphocytosis

Notes on the diagnosis of acute leukemia

Role of the cytometry laboratory in the autologous bone marrow transplantation

The basophilic degranulation test, and its role in allergy diagnostics

Cytometric analysis of broncho-alveolar lavage fluid

Cytometric diagnosis of paroxysmal nocturnal hemoglobinuria

Learning Achievements (Dublin Descriptors)

Knowledge and understanding. The student should demonstrate knowledge of the topics listed in the program and be able to understand the relationships between the various information related to them.

Applied knowledge and understanding. The student should prove to be able to interpret the data in his/her possession in order to understand the diagnostic problem.

Autonomy of judgment. The student should prove to be able to process the data to get autonomously a reasoned solution to the diagnostic problem

Communication skills. The student should prove to be able to communicate clearly and consequentially the diagnostic process followed, and the relevance of the final result.

Ability to learn. The student should prove to be able to coordinate the information progressively received during the course, using them logically in a global approach.

Teaching Material

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Supporting Activities

The teaching material prepared by the lecturer (such as for instance slides, lecture notes, exercises) and specific communications from the lecturer can be found, together with other supporting activities, inside the Moodle platform

Didactics, Attendance, Course Books and Assessment


Lectures and practical computer exercises with simulated diagnosis of typical cases.


Mandatory attendance

Course books

The Course Book consists of the material provided by the teacher and accessible in Moodle. This material consists of the book "Notes of Diagnostic Cytometry" and of the slides projected in class.

As a reference material, and only for the topics discussed in class, we propose the text "Cytometric Diagnosis of Hematologic Neoplasms", Ortolani, Editrice Quattroventi, also uploaded in Moodle.

Still only for topics discussed in class, another consultation text is "Cellular Diagnostics: Basic Principles, Methods and Clinical Applications of Flow Cytometry", Sack, Tarnok, Rothe eds, Karger 2009. In case of problems in the text availability, please contact the teacher.


Final examination consisting of the computer analysis of a typical case. The evaluation of the candidate will be expressed as suitable or not suitable.

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