LABORATORY OF MOLECULAR BIOTECHNOLOGY
LABORATORIO DI BIOTECNOLOGIE MOLECOLARI
| A.Y. | Credits |
|---|---|
| 2019/2020 | 4 |
| Lecturer | Office hours for students | |
|---|---|---|
| Stefano Amatori |
| Teaching in foreign languages |
|---|
|
Course partially taught in a foreign language
English
This course is taught partially in Italian and partially in a foreign language. Study materials can be provided in the foreign language and the final exam can be taken in the foreign language. |
Assigned to the Degree Course
| Date | Time | Classroom / Location |
|---|
| Date | Time | Classroom / Location |
|---|
Program
1 - Cell biology techniques
- Safety and sterility in cell culture laboratories
- Instrumentation in cell cultures (cell culture hood and incubator)
- Cell culture media and consumables
- Cell counting strategies
- Exercise: thawing, counting, maintenance and freezing of immortalized human cell lines
2 - Fluorescence microscopy techniques
- Fluorescence analysis
- Quantitative and qualitative determination
- The fluorescence microscope
- Exercise: cellular localization of a protein by immunofluorescence
3 - Flow cytometry techniques
- The cytofluorimeter
- Flow cytometry methods
- Exercise: cytofluorimetric analysis of the cycle of tumor cells subjected to treatment with potential anticancer molecules
4 - DNA sequencing techniques
- Sanger technique
- The Sanger sequencer
- Next generation sequencing (NGS)
- NGS sequencers
- Exercise: Sanger sequencing and presentation of the NGS Ion S5 sequencing system
5 - Epigenetic techniques
- DNA methylation analysis
- Chromatin analysis: chromatin immunoprecipitation (ChIP)
Teaching Material
The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it
| « back | Last update: 31/07/2019 |
