Università degli Studi di Urbino Carlo Bo / Portale Web di Ateneo


DIAGNOSTIC AND THERAPEUTIC BIOTECHNOLOGIES
BIOTECNOLOGIE DIAGNOSTICHE E TERAPEUTICHE

A.Y. Credits
2019/2020 6
Lecturer Email Office hours for students
Luca Galluzzi To be agreed by phone or email. Two hours per week will be reserved for ERASMUS assistance
Teaching in foreign languages
Course partially taught in a foreign language English
This course is taught partially in Italian and partially in a foreign language. Study materials can be provided in the foreign language and the final exam can be taken in the foreign language.

Assigned to the Degree Course

Biotechnology (L-2)
Curriculum: PERCORSO COMUNE
Giorno Orario Aula
Giorno Orario Aula

Learning Objectives

The course aims to provide students with knowledge on the theoretical bases and applications of biotechnology techniques in the fields of diagnostics and drugs production, with particular reference to nucleic acids-based methods and the use of recombinant DNA technology.

Program

1. nucleic acid amplification in molecular diagnostics
1.1 PCR-based methods 
1.2 non PCR-based methods
2. Detection of gene mutations 
2.1 gene mutation nomenclature 
2.2 methods for detection of gene mutations (hybridization-based methods, polymerization-based methods, cleavage methods)
3. DNA sequencing in molecular diagnostics
3.1 Pyrosequencing
3.2 Bisulfite DNA sequencing
4. DNA polymorphisms and human identification
4.1 types of polymorphisms
4.2 RFLP typing
4.3 STR typing
4.4 Y-STR
4.5 Engraftment testing
4.6 Mitochondrial DNA polymorphisms
5. Detection and identification of microorganisms 
5.1 sample collection, preparation and quality controls
5.2 targets of molecular-based tests
5.3 resistance to antimicrobial agents
5.4 typing methods
6. Molecular detection of inherited diseases
6.1 molecular diagnosis of single-gene disorders
6.2 single-gene disorders with nonclassical patterns of inheritance 
7. Diagnostic targets in molecular oncology 
7.1 mutations in solid tumors
7.2 microsatellite instability 
7.3 loss of heterozygosity
7.4 gene rearrangements in leukemia and lymphoma 
8. Chemical oligonucleotide synthesis 
9. DNA mutagenesis techniques
10.  Microbial production of therapeutic agents 
10.1 hormones
10.2 cytokines
10.3 blood proteins
10.4 enzymes
11. Monoclonal antibodies 
11.1 Monoclonal antibodies engineering (murine, chimeric, humanized and human antibodies, scFv)
11.2 therapeutic and diagnostic applications of monoclonal antibodies 
12. Production of pharmaceutical proteins by transgenic animals 
13. gene therapy
13.1 vectors 
13.2 Haematopietic stem cells applications in gene therapy

 

Learning Achievements (Dublin Descriptors)

D1 - Knowledge and ability of comprehension. The student must demonstrate to have gained the knowledge inherent to the teaching program, with special reference to molecular diagnostics techniques and to techniques for DNA and cells manipulation aimed to the production of recombinant proteins.

D2 - Ability to apply knowledge and comprehension The student must be able to implement/use these knowledges in the field of biotechnology, to describe and/or hypothesize experimental approaches aimed to produce molecular diagnostics test and therapeutics.

D3 - Autonomy of judgement The student will be able to critically evaluate the methods/strategies used for diangostics or production of therapeutic agents.

D4 - Communication skills The student must acquire adequate scientific language skills by taking a participatory and critical attitude to the topics of the lectures.

D5 - Learning skill. The student will be able to build his own learning path, using learning materials fournished by the teacher or autonomously acquired.

Teaching Material

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Supporting Activities

The slides will be made available online (moodle platform). Scientific articles (reviews) could be indicated during classes.


Didactics, Attendance, Course Books and Assessment

Didactics

Lectures

Course books
  • Buckingham L. "Molecular diagnostics. Fundamentals, methods and clinical applications". 2nd edition. 2011. Ed. F.A. DAVIS
  • Calabrò ML. "Compendio di biotecnologie farmaceutiche" 2008. Ed. Edises
  • Daan J. A. Crommelin, ‎Robert D. Sindelar, ‎Bernd Meibohm. Pharmaceutical Biotechnology: Fundamentals and Applications. 4th edition 2013. SPRINGER
     
  • Assessment

    Oral examination (3-4 questions regarding different topics). During the examination the student must demonstrate: that it has acquired full mastery of the concepts covered during the course; to be able to make logical connections between the techniques learned and their biotechnological applications; to be able to illustrate the key concepts in a limited time and using appropriate scientific language.

    Additional Information for Non-Attending Students

    Course books
  • Buckingham L. "Molecular diagnostics. Fundamentals, methods and clinical applications". 2nd edition. 2011. Ed. F.A. DAVIS
  • Calabrò ML. "Compendio di biotecnologie farmaceutiche" 2008. Ed. Edises
  • Daan J. A. Crommelin, ‎Robert D. Sindelar, ‎Bernd Meibohm. Pharmaceutical Biotechnology: Fundamentals and Applications. 4th edition 2013. SPRINGER
     
  • Assessment

    Oral examination (3-4 questions regarding different topics). During the examination the student must demonstrate: that it has acquired full mastery of the concepts covered during the course; to be able to make logical connections between the techniques learned and their biotechnological applications; to be able to illustrate the key concepts in a limited time and using appropriate scientific language.

    « back Last update: 12/07/19

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