BASIC AND APPLIED CYTOMETRY MOD.2
BASIC AND APPLIED CYTOMETRY MOD.2
| A.Y. | Credits |
|---|---|
| 2022/2023 | 3 |
| Lecturer | Office hours for students | |
|---|---|---|
| Stefano Papa |
Assigned to the Degree Course
| Date | Time | Classroom / Location |
|---|
| Date | Time | Classroom / Location |
|---|
Learning Objectives
The course aims to train PhD students on an advanced cellular analysis technology: flow cytometry.
In this sense, the course will develop into basic lessons that will allow the doctoral student to understand the rudiments of this technology, in particular the physicochemical bases that allow to study cells and subcellular particles using fluorochromes as specific dyes or adhering to specific vectors (eg. Monoclonal Antibodies) using multiple fluorescence analysis.
Application lessons will follow which will cover the two major fields of application;
a) cell biology (study of cell kinetics, cell stress phenomena, apoptosis, study of cytoplasmic organelles)
b) the study of the phenotype of normal and pathological hematopoietic cells
Program
Physical basis of light (Lasers) and light detection systems (PMT and Photodiodes)
Fluid physics, hydrodynamic structure of the flow cytometer.
Optical bench, pulse generation and their digitization, biparametric dot-plot matrix for the study of signals, gating systems of the clusters highlighted in the matrices and the "Boolean gating" for identifying subsets.
Compensation of fluorescences on the basis of the emission spectrum of fluorochromes
Mechanical and electrical cytometric sorting
Bridging Courses
It is advisable to follow module 1 and module 2 at the same time
Learning Achievements (Dublin Descriptors)
Once the student has followed the course in its 2 modules, he will be able to evaluate the possibility of using this technology and the possible benefits that the application of this investigation procedure will bring to his research project. Furthermore, he will have had the opportunity to see and understand in detail the clinical, immunological and oncological applications of flow cytometry.
Teaching Material
The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it
Supporting Activities
Upon agreement with the doctoral student, attendance at the cytometry laboratory will be possible
Teaching, Attendance, Course Books and Assessment
- Attendance
At least 2 out of 3 lessons must be followed by the student
- Course books
The specialist text for this topic of the PhD student's training:
"Flow cytometry" by Claudio Ortolani, Stefano Papa et al. Ed: EdiErmes Milan 2020
- Assessment
A short quiz on the activities carried out will be given at the end of the whole module.
- Disabilità e DSA
Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.
A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.
| « back | Last update: 05/11/2022 |
