During the Analytical Chemistry course, the student will learn some of the most advanced analytical techniques. The essential instrumental methods will be covered as well, in particular those for modern and advanced biological applications. Technical details and applications will also be considered. Specific emphasis will be given to chromatography and mass spectrometry.

A brief review of some basic concepts: measurement units of concentration. Preparation of solutions and related dilutions. Validation of analytical methods: Measurement error: Significant figures. Errors and their identification and minimization. Precision and accuracy. Calibration. Pearson coefficient. Least squares method. Standardization procedures. Sample preparation techniques. Extraction and pre-concentration. Recovery efficiency.

Chromatographic methods: Distribution coefficient. Column chromatography. The chromatogram and chromatographic parameters. Chromatographic resolution and efficiency. Band broadening. Van Deemter equation.

High-performance liquid chromatography: Stationary phases and mobile phases. Column types. UHPLC and other high-efficiency techniques. Separation mechanisms: adsorption, distribution, ion exchange, dimensional exclusion. Ion chromatography. HPLC injector. HPLC detectors: RI, UV-Vis, Fluorescence; Electrochemical detectors.

Mass spectrometry: Ionization techniques: electron ionization, chemical ionization, electrospray ionization (ESI), matrix effects, multicharged ions. General characteristics of a mass spectrum. Mass spectrometric resolution. Characteristic fragmentations. Isotope clusters. Mass analyzers: quadrupole (Q), ion trap (QIT), time of flight (TOF). Chromatography-mass spectrometry coupling: HPLC-MS. Instrumental configurations and operating modes. HPLC-MS interfaces. Use of the mass spectrometer in the analytical field: SIM, Scan, MSMS.

Examples and applications.

Knowledge and understanding skills. The student will have to know the main analytical techniques available for qualitative and quantitative analysis. He/she should also have to know which are the most important substance classes in biological samples. The student should learn the techniques available for sampling, conservation, and qualitative and quantitative analysis of chemical components and how to apply them to the actual samples. The student should be able to determine what instrumentation is best suited to the needs of the sample. These skills will be verified through written questions.

Ability to apply knowledge and understanding. The student should be able to correctly use the terminology related to analytical chemistry. He will need to master the concentration units. He will need to know how to choose between sampling techniques, sample handling, and analysis to be applied to a biological sample. These skills will be verified through written questions.

Judgment autonomy. The student must be able to make the right decisions on how to deal with an issue related to the analysis of a biological sample.

Communicative Skills. The student should be able to clearly describe the analytical techniques studied with appropriate terms and appropriate examples.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Presentation slides.

Attendance

Students who have attended at least 70% of lessons.

Course books

Daniel C. Harris - Chimica Analitica Quantitativa - edizione 2017 - Zanichelli, Cozzi, Protti, Ruaro - Elementi di analisi chimica strumentale - Seconda edizione - Zanichelli, Holler, Skoog, Crouch - Chimica analitica strumentale - Seconda edizione - EdiSES, Dong - Modern HPLC for Practicing Scientists - Wiley, Dass - Fundamentals of Contemporary Mass Spectrometry - Wiley, Cappiello - Advances in LC-MS Instrumetation - Elsevier

Assessment

Written test with six exercises and open questions on several topics taken from the course program. A 45-minute time is allowed to complete the test using a form with the six questions and enough space for the answers. The evaluation considers the sum of all answers and their overall quality, emphasizing the correct use of scientific terminology.

Disabilità e DSA

Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.

A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.

Attendance

Students who have attended at least 70% of lessons.

Course books

Daniel C. Harris - Chimica Analitica Quantitativa - edizione 2017 - Zanichelli, Cozzi, Protti, Ruaro - Elementi di analisi chimica strumentale - Seconda edizione - Zanichelli, Holler, Skoog, Crouch - Chimica analitica strumentale - Seconda edizione - EdiSES, Dong - Modern HPLC for Practicing Scientists - Wiley, Dass - Fundamentals of Contemporary Mass Spectrometry - Wiley, Cappiello - Advances in LC-MS Instrumetation - Elsevier

Assessment

Written test with six exercises and open questions on several topics taken from the course program. A 45-minute time is allowed to complete the test using a form with the six questions and enough space for the answers. The evaluation considers the sum of all answers and their overall quality, emphasizing the correct use of scientific terminology.

Disabilità e DSA

Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.

A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.