CYTOHISTOLOGY AND CYTOMETRY - UNIT MICROSCOPY AND CYTOMETRY
CITOISTOLOGIA E CITOMETRIA MODULO DI MICROSCOPIA E CITOMETRIA
A.Y. | Credits |
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2015/2016 | 6 |
Lecturer | Office hours for students | |
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Stefano Papa |
Assigned to the Degree Course
Date | Time | Classroom / Location |
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Learning Objectives
The didactic route of the entire course is built u with a number of biological matters with the aim to obtain a clear background on techniques and applied methodologies for the morphological analysis of different human body systems. In this direction the didactic route provide at first a setting on methodologies for the characterization of the sample in Citohistology and Cytochemistry/Histchemistry units, then this unit, Cytometry, faces subjects related to instrumentations for the dynamic automated cell analysis at high resolution and its related methodologies for the phenotypic study of cells at the molecular level, the cell differentiation process and some cellular metabolic processes.
Program
Optics: light diffusion – scattering, light absorbance and fluorescence, fluorochromes.
Flow cytometer structure and sorter function
Flow acquisition and analysis software, histogram and dot-plot analysis
Application in cell kinetics: DNA measure, cell kinetics, proliferation probes, protocol for DNA/BrdU analysis
Application in Immunology: qualitative and quantitative phenotype, cell immunology and subset analysis (Cluster)
Application in Cell Function: membrane potential, apoptosis, cell-to-cell interaction (Cross talk), mitochondria and chromosome analysis.
Flow Instrumentation: FACSaria, FACSCanto, Coulter Navios, MoFlow.
Learning Achievements (Dublin Descriptors)
The student must show mastery of basic knowledge related to geometrical optics and optical physics elements necessary for a proper understanding of the operation of the two types that characterize the instrumental teaching.
The student must show an understanding of the concepts and theories provided by the course; It will provide examples of morphometric and cytometric analysis identifying strengths and weaknesses of the various instruments depending on the field of biological investigation.
The student must show the requisite skills and concepts and to use the knowledge gained by demonstrating to reason according to the specific logic of the discipline. It will be able in particular to identify the most appropriate methodologies appropriate to the context in question; simulate a protocol of investigation; envisage some possibility of intervention by identifying the tool best suited to the analysis cytology.
Teaching Material
The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it
Supporting Activities
The educational material is present on Google Drive, accessible and downloadable from students through credentials
Teaching, Attendance, Course Books and Assessment
- Teaching
The teaching consists of lectures and laboratory exercises teaching
- Attendance
We recommend the attendance of lectures to get a good learning of the subject
- Course books
Texts (handouts) are accessible on Google Drive with the PDF lessons
- Assessment
It will carry out surveys of learning evaluation during the first cycle (microscopy) and second cycle (cytometry) to assess the level of learning on the entire course program.
The methods of assessment are to only oral exam
- Disabilità e DSA
Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.
A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.
Additional Information for Non-Attending Students
- Attendance
We strongly recommend the attendance of lectures to get a good learning of the subject
- Course books
Texts (handouts) are accessible on Google Drive with the PDF lessons
- Assessment
It will carry out surveys of learning evaluation during the first cycle (microscopy) and second cycle (cytometry) to assess the level of learning on the entire course program.
The methods of assessment are to only oral exam
- Disabilità e DSA
Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.
A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.
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