The course aims to train PhD students on an advanced cellular analysis technology: flow cytometry.

In this sense, the course will develop into basic lessons that will allow the doctoral student to understand the rudiments of this technology, in particular the physicochemical bases that allow to study cells and subcellular particles using fluorochromes as specific dyes or adhering to specific vectors (eg. Monoclonal Antibodies) using multiple fluorescence analysis. Finally, Flow citometric cell sorting will be discussed. Lessons will cover the two major fields of application descending from the program: a) cell biology (study of cell kinetics, cell stress phenomena, apoptosis, study of cytoplasmic organelles); b) Cell Sorting 

Physical basis of light (Lasers) and light detection systems (PMT and Photodiodes)

Fluid physics, hydrodynamic structure of the flow cytometer.

Optical bench, pulse generation and their digitization, biparametric dot-plot matrix for the study of signals, gating systems of the clusters highlighted in the matrices and the "Boolean gating" for identifying subsets.

Compensation of fluorescences on the basis of the emission spectrum of fluorochromes

Mechanical and electrical cytometric sorting

The student, once he/she has followed the course in its 2 modules, will be able to assess the possibility of using this technology and the possible benefits that the application of this investigation procedure may bring to his/her research project.

Students on the course will be required to demonstrate: 

D1. Knowledge and ability to understand: Know the possible applications of cytometry.

D2. Ability to apply knowledge and understanding: Understand and use the specific language of the discipline and to exploit  additional bibliographic information.  

D3. Ability to judge: to acquire the indispensable and adequate competences for defining the utility, in their own project, of a specific flow cytometer (i.e its specific optic bench top)

D4. Communication skills: to acquire critical and participative attitudes to the topics of the lectures.

D5. Learning ability: ability to design an adequate cytometric panel depending on the tissue and the goal of use.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Upon agreement with the doctoral student, attendance at the cytometry laboratory will be possible

Attendance

Both of the 2 lessons must be followed by the student

Course books

For PhD students that want to go into detail, the specialist text for this topic is:"Flow cytometry" by Claudio Ortolani, Stefano Papa et al. Ed: EdiErmes Milan 2020

Teaching

Idem Frequentanti 

Attendance

Idem Frequentanti

Course books

For PhD students that want to go into detail, the specialist text for this topic is:"Flow cytometry" by Claudio Ortolani, Stefano Papa et al. Ed: EdiErmes Milan 2020